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Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica
Our results provide direct evidence of how PVC cargoes are loaded and delivered directly into mammalian macrophages.
2021-06-25 -
Structures of human dual oxidase 1 complex in low-calcium and high-calcium states
In the presence of calcium ions, intracellular EF-hand modules might enhance the catalytic activity of DUOX by stabilizing the dehydrogenase domain in a conformation that allows electron transfer.
2021-01-08 -
Structure, function and pharmacology of human itch receptor complexes
Collectively, our results provide a structural basis for the sensing of cationic allergens by MRGPRX2, potentially facilitating the rational design of therapies to prevent unwanted pseudoallergic reactions.
2021-11-17 -
Structural insights into TSC complex assembly and GAP activity on Rheb
Our study reveals mechanisms of TSC complex assembly and GAP activity.
2021-01-12 -
Asymmetric Structures and Conformational Plasticity of the Uncleaved Full-Length Human Immunodeficiency Virus Envelope Glycoprotein Trimer
Selective pressure to maintain flexibility in the precursor of functional Env allows the uncleaved Env to sample asymmetric conformations that potentially skew host antibody responses toward pNAbs.
2021-11-23 -
Structure of the mitochondrial TIM22 complex from yeast
To address some of the fundamental questions in the field, we determined the electron cryo-microscopy (cryo-EM) structure of the endogenous TIM22 complex from S. cerevisiae.
2020-09-11 -
Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding
Paused or collided ribosomes are associated with specific chaperones, which can aid in the cotranslational folding of the nascent peptides.
2021-01-05 -
Structural insight into the mechanism of energy transfer in cyanobacterial phycobilisomes
These results provide insights into the organization of chromophores and the mechanisms of EET within cyanobacterial PBS.
2021-09-17 -
Cryo-EM structures of human GMPPA–GMPPB complex reveal how cells maintain GDP-mannose homeostasis
We conclude that GMPPA acts as a cellular sensor to maintain mannose homeostasis through allosterically regulating GMPPB.
2021-05-13 -
Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents
SA55+SA58 potently neutralizes ACE2-utilizing sarbecoviruses, including circulating Omicron variants, and could serve as broad SARS-CoV-2 prophylactics to offer long-term protection, especially for individuals who are immunocompromised or with high-risk comorbidities.
2020-12-20 -
Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies
Our results inform the development of antibody-based drugs and vaccines against Omicron and future variants.
2021-12-23 -
Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase
This study elucidates the versatility of Dbf4 in regulating the ordered multisite phosphorylation of the MCM-DH by Cdc7 kinase during helicase activation.
2022-03-16 -
Structural identification of vasodilator binding sites on the SUR2 subunit
These KATP openers synergize with Mg-nucleotides to stabilize SUR2 in the NBD-dimerized occluded state to activate the channel.
2022-05-13 -
Structural insights into how GlcNAc-1-phosphotransferase directs lysosomal protein transport
These results provide important insights into GlcNAc-1-phosphotransferase function and related diseases.
2022-03-15 -
Structural mechanism of human TRPC3 and TRPC6 channel regulation by their intracellular calciumbinding sites
Furthermore, structures of human TRPC6 in complex with two chemically distinct inhibitors bound at different ligand-binding pockets reveal different conformations of the transmembrane domain, providing templates for further structure-based drug design targeting TRPC6-related diseases such as FSGS.
2022-03-16 -
Identification of ASPDH as a novel NAADP-binding protein
In contrast, recombinantly expressed Jupiter microtubule-associated homolog 2 (JPT2) and SM-like protein LSM12, two proteins previously identified as NAADP-receptors, show no binding in ITC experiments.
2022-07-13 -
Structure and mechanism of NALCN-FAM155A-UNC79-UNC80 channel complex
Our work defines the structural mechanism of NALCN modulation by UNC79 and UNC80.
2022-05-12 -
Tuning the Size of Large Dense-Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid–Liquid Phase Separation
In conclusion, it is shown that SgII is a unique intravesicular matrix protein undergoing liquid–liquid phase separation, and present novel insights into how SgII determines LDCV size and the quantal neurotransmitter release.
2022-07-27 -
Structure of human phagocyte NADPH oxidase in the resting state
Structural analysis suggests that the cytosolic factors might activate the NOX2-p22 complex by stabilizing the DH in a productive docked conformation.
2023-12-18 -
Mutations on RBD of SARS-CoV-2 Omicron variant result in stronger binding to human ACE2 receptor
The mutations at the ACE2-RBD interface enhance the tight binding by increasing hydrogen bonding interaction and enlarging buried solvent accessible surface area.
2022-01-29